Genomic Comparison of Non-Typhoidal Salmonella enterica Serovars Typhimurium, Enteritidis, Heidelberg, Hadar and Kentucky Isolates from Broiler Chickens

PLoS One. 2015 Jun 17;10(6):e0128773. doi: 10.1371/journal.pone.0128773. eCollection 2015. Dhanani AS1, Block G2, Dewar K3, Forgetta V4, Topp E5, Beiko RG1, Diarra MS2. Abstract BACKGROUND: Non-typhoidal Salmonella enterica serovars, associated with different foods including poultry products, are important causes of bacterial gastroenteritis worldwide. The colonization of the chicken gut by S. enterica could result in […]

Effects of Tributyrin on Intestinal Energy Status, Antioxidative Capacity and Immune Response to Lipopolysaccharide Challenge in Broilers

Asian-Australas J Anim Sci. 2015 Dec;28(12):1784-93. doi: 10.5713/ajas.15.0286. Effects of Tributyrin on Intestinal Energy Status, Antioxidative Capacity and Immune Response to Lipopolysaccharide Challenge in Broilers. Li J1, Hou Y1, Yi D1, Zhang J1, Wang L1, Qiu H1, Ding B2, Gong J3. Author information Abstract This study was carried out to investigate the effects of tributyrin […]

Modulation of cytokine gene expression by selected Lactobacillus isolates in the ileum, caecal tonsils and spleen of Salmonella-challenged broilers

Avian Pathol. 2015;44(6):463-9. doi: 10.1080/03079457.2015.1086725. Modulation of cytokine gene expression by selected Lactobacillus isolates in the ileum, caecal tonsils and spleen of Salmonella-challenged broilers. Hu JL1,2, Yu H2, Kulkarni RR3, Sharif S3, Cui SW2, Xie MY1, Nie SP1, Gong J2. Author information Abstract Probiotics have been used to control Salmonella colonization in the chicken intestine. […]

Phytogenic compounds as alternatives to in-feed antibiotics: potentials and challenges in application

Pathogens. 2015 Mar 23;4(1):137-56. doi: 10.3390/pathogens4010137. Phytogenic compounds as alternatives to in-feed antibiotics: potentials and challenges in application. Yang C1, Chowdhury MA2, Huo Y3, Gong J4. Author information Abstract This article summarizes current experimental knowledge on the efficacy, possible mechanisms and feasibility in the application of phytogenic products as feed additives for food-producing animals. Phytogenic […]

In ovo delivery of CpG DNA reduces avian infectious laryngotracheitis virus induced mortality and morbidity.

Viruses. 2015 Apr 8;7(4):1832-52. doi: 10.3390/v7041832. In ovo delivery of CpG DNA reduces avian infectious laryngotracheitis virus induced mortality and morbidity. Thapa S1, Cader MS2, Murugananthan K3, Nagy E4, Sharif S5, Czub M2, Abdul-Careem MF6. Author information Abstract Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, […]

In ovo delivery of Toll-like receptor 2 ligand, lipoteichoic acid induces pro-inflammatory media-tors reducing post-hatch infectious laryngotracheitis virus infection.

Vet Immunol Immunopathol. 2015 Apr 15;164(3-4):170-8. doi: 10.1016/j.vetimm.2015.02.006. Epub 2015 Feb 20. In ovo delivery of Toll-like receptor 2 ligand, lipoteichoic acid induces pro-inflammatory mediators reducing post-hatch infectious laryngotracheitis virus infection. Thapa S1, Nagy E2, Abdul-Careem MF3. Abstract Toll-like receptor (TLR) ligands are pathogen associated molecular patterns (PAMPs) recognized by the TLRs resulting in induction […]

Toll-like receptor 2 ligand, lipoteichoic acid is inhibitory against infectious laryngotracheitis virus infection in vitro and in vivo.

Dev Comp Immunol. 2015 Jan;48(1):22-32. doi: 10.1016/j.dci.2014.08.011. Epub 2014 Sep 6. Haddadi S1, Thapa S1, Kameka AM1, Hui J1, Czub M1, Nagy E2, Muench G3, Abdul-Careem MF4. Abstract Lipoteichoic acid (LTA) is one of the pathogen associated molecular patterns (PAMPs) that activates toll-like receptor (TLR)2-cluster of differentiation (CD)14 signalling pathway. This recognition elicits antiviral responses […]

Cytokine Gene Expression in Lung Mononuclear Cells of Chickens Vaccinated with Herpesvirus of Turkeys and Infected with Marek’s Disease Virus

Viral Immunol. 2015 Nov;28(9):538-43. doi: 10.1089/vim.2015.0054. Epub 2015 Oct 8. Parvizi P1, Brisbin JT1, Read LR1, Sharif S1. Abstract Marek’s disease virus (MDV) enters the chicken host through the respiratory system. However, little is known about the host immune responses induced by MDV in the lungs. To characterize these responses, chickens were vaccinated with herpesvirus […]

Reduction of avian influenza virus shedding by administration of Toll-like receptor ligands to chickens

Vaccine. 2015 Sep 11;33(38):4843-9. doi: 10.1016/j.vaccine.2015.07.070. Epub 2015 Aug 1. Barjesteh N1, Shojadoost B1, Brisbin JT2, Emam M1, Hodgins DC1, Nagy É1, Sharif S3. Abstract Avian influenza viruses (AIV) are of concern to the poultry industry. Outbreaks of AIV highlight the urgent need for effective control measures. Prophylactic strategies should be explored that rapidly elicit […]

Systemic immune responses to an inactivated, whole H9N2 avian influenza virus vaccine using class B CpG oligonucleotides in chickens

Vaccine. 2015 Jul 31;33(32):3947-52. doi: 10.1016/j.vaccine.2015.06.043. Epub 2015 Jun 16. Singh SM1, Alkie TN2, Hodgins DC3, Nagy É4, Shojadoost B5, Sharif S6. Abstract Commercial vaccines against avian influenza viruses (AIV) in chickens consist mainly of inactivated AIV, requiring parenteral administration and co-delivery of an adjuvant. Limitations in T helper 1 or T helper 2 biased […]

Evaluation of a polysaccharide conjugate vaccine to reduce colonization by Campylobacter jejuni in broiler chickens

BMC Res Notes. 2015 Jun 2;8:204. doi: 10.1186/s13104-015-1203-z. Hodgins DC1, Barjesteh N2, St Paul M3,4, Ma Z5, Monteiro MA6, Sharif S7. Abstract BACKGROUND: Campylobacter jejuni is a leading bacterial cause of food-borne illness in humans. Symptoms range from mild gastroenteritis to dysentery. Contaminated chicken meat is the most common cause of infection. Broiler chickens become […]

Rapid generation of fowl adenovirus 9 vectors

J Virol Methods. 2015 Oct;223:75-81. doi: 10.1016/j.jviromet.2015.07.017. Epub 2015 Jul 31. Pei Y1, Griffin B1, de Jong J1, Krell PJ2, Nagy É3. Abstract Fowl adenoviruses (FAdV) have the largest genomes of any fully sequenced adenovirus genome, and are widely considered as excellent platforms for vaccine development and gene therapy. As such, there is a strong […]

On-farm comparison of keel fracture prevalence and other welfare indicators in conventional cage and floor-housed laying hens in Ontario, Canada

Poult Sci. 2015 Apr;94(4):579-85. doi: 10.3382/ps/pev039. Epub 2015 Feb 22. Petrik MT1, Guerin MT2, Widowski TM3. Abstract The objective of this study was to compare the flock-level prevalence of healed keel bone fractures and to benchmark other indicators of well-being in laying hens housed in conventional cages and single-tier floor housing systems at several points […]

Soft perches in an aviary system reduce incidence of keel bone damage in laying hens

PLoS One. 2015 Mar 26;10(3):e0122568. doi: 10.1371/journal.pone.0122568. eCollection 2015. Stratmann A1, Fröhlich EK2, Harlander-Matauschek A3, Schrader L4, Toscano MJ1, Würbel H1, Gebhardt-Henrich SG1. Abstract Keel bone fractures and deviations are one of the major welfare and health issues in commercial laying hens. In non-cage housing systems like aviaries, falls and collisions with perches and other […]

2015 Publications

Selected Publications Genomic Comparison of Non-Typhoidal Salmonella enterica Serovars Typhimurium, Enteritidis, Heidelberg, Hadar and Kentucky Isolates from Broiler Chickens Effects of Tributyrin on Intestinal Energy Status, Antioxidative Capacity and Immune Response to Lipopolysaccharide Challenge in Broilers Modulation of cytokine gene expression by selected Lactobacillus isolates in the ileum, caecal tonsils and spleen of Salmonella-challenged broilers […]

Control of the reproductive axis: Balancing act between stimulatory and inhibitory inputs.

Poult Sci. 2015 Apr;94(4):810-5. doi: 10.3382/ps/peu042. Epub 2015 Jan 30. Bédécarrats GY1. Abstract As for most vertebrates, reproduction in poultry is controlled by an integrated axis [the hypothalamo-pituitary-gonadal (HPG) axis]. External and internal cues are integrated at the level of the hypothalamus to initiate gonadal recruitment and control the subsequent reproductive cycle. Until recently, it […]

From the Macro to the Micro: Gel Mapping to Differentiate between Sporozoites of Two Immu-nologically Distinct Strains of Eimeria maxima (Strains M6 and Guelph).

PLoS One. 2015 Dec 7;10(12):e0143232. doi: 10.1371/journal.pone.0143232. eCollection 2015. El-Ashram S1,2,3,4, Yin Q1,2, Liu H5, Al Nasr I6, Liu X1,2, Suo X1,2, Barta J4. Abstract Two immunologically distinct strains of E. maxima were examined in this study: the M6 strain and the Guelph strain. The differential expression between the sporozoites of the two strains of […]

Molecular phylogenetics of eimeriid coccidia (Eimeriidae, Eimeriorina, Apicomplexa, Alveolata): A preliminary multi-gene and multi-genome approach.

Parasitol Res. 2015 Nov;114(11):4149-60. doi: 10.1007/s00436-015-4646-1. Epub 2015 Aug 29. Ogedengbe JD1, Ogedengbe ME1, Hafeez MA1, Barta JR2. Abstract Coccidia possess three distinct genomes: nuclear, mitochondrial, and plastid. Sequences from five genes located on these three genomes were used to reconstruct the phylogenetic relationships of members of the phylum Apicomplexa: 18S rDNA sequences from the […]

A linear mitochondrial genome of Cyclospora cayetanensis (Eimeriidae, Eucoccidiorida, Coc-cidiasina, Apicomplexa) suggests the ancestral start position within mitochondrial genomes of eimeriid coccidia.

Int J Parasitol. 2015 May;45(6):361-5. doi: 10.1016/j.ijpara.2015.02.006. Epub 2015 Mar 23. Ogedengbe ME1, Qvarnstrom Y2, da Silva AJ3, Arrowood MJ2, Barta JR4. Abstract The near complete mitochondrial genome for Cyclospora cayetanensis is 6184 bp in length with three protein-coding genes (Cox1, Cox3, CytB) and numerous lsrDNA and ssrDNA fragments. Gene arrangements were conserved with other […]

Simultaneous identification and DNA barcoding of six Eimeria species infecting turkeys using PCR primers targeting the mitochondrial cytochrome c oxidase subunit I (mtCOI) locus

Parasitol Res. 2015 May;114(5):1761-8. doi: 10.1007/s00436-015-4361-y. Epub 2015 Feb 14. Hafeez MA1, Shivaramaiah S, Dorsey KM, Ogedengbe ME, El-Sherry S, Whale J, Cobean J, Barta JR. Abstract Species-specific PCR primers targeting the mitochondrial cytochrome c oxidase subunit I (mtCOI) locus were generated that allow for the specific identification of the most common Eimeria species infecting […]

Shedding of live Eimeria vaccine progeny is delayed in chicks with delayed access to feed after vaccination

Vet Parasitol. 2015 Mar 15;208(3-4):242-5. doi: 10.1016/j.vetpar.2015.01.009. Epub 2015 Jan 17. Price KR1, Freeman M2, Van-Heerden K3, Barta JR4. Abstract Hatching, processing and transportation result in inevitable delays before chicks are placed into brooding and receive their first feed and drinking water after hatching. To determine if delayed access to feed for different durations following […]

Genotyping of infectious bronchitis viruses identified in Canada between 2000 and 2013

Avian Pathol. 2014;43(3):264-8. doi: 10.1080/03079457.2014.916395. Epub 2014 May 16. Martin EA1, Brash ML, Hoyland SK, Coventry JM, Sandrock C, Guerin MT, Ojkic D. Abstract Infectious bronchitis virus (IBV) was detected in 185 samples originating from chicken flocks of various commodity groups in Canada. Flocks with clinical signs such as respiratory challenge, sudden death, egg production […]

Antibiotics in Canadian poultry productions and anticipated alternatives

Front Microbiol. 2014 Jun 17;5:282. doi: 10.3389/fmicb.2014.00282. eCollection 2014. Diarra MS1, Malouin F2. Abstract The use of antibiotics in food-producing animals has significantly increased animal health by lowering mortality and the incidence of diseases. Antibiotics also have largely contributed to increase productivity of farms. However, antibiotic usage in general and relevance of non-therapeutic antibiotics (growth […]

Characterization of Extraintestinal Pathogenic Escherichia coli isolated from retail poultry meats from Alberta, Canada.

Int J Food Microbiol. 2014 May 2;177:49-56. doi: 10.1016/j.ijfoodmicro.2014.02.006. Epub 2014 Feb 21. Aslam M1, Toufeer M1, Narvaez Bravo C2, Lai V3, Rempel H3, Manges A4, Diarra MS5. Abstract Extraintestinal Pathogenic Escherichia coli (ExPEC) have the potential to spread through fecal waste resulting in the contamination of both farm workers and retail poultry meat in […]

Selected lactic acid-producing bacterial isolates with the capacity to reduce Salmonella translocation and virulence gene expression in chickens

PLoS One. 2014 Apr 11;9(4):e93022. doi: 10.1371/journal.pone.0093022. eCollection 2014. Yang X1, Brisbin J2, Yu H1, Wang Q1, Yin F1, Zhang Y1, Sabour P1, Sharif S2, Gong J1. Abstract BACKGROUND: Probiotics have been used to control Salmonella colonization/infection in chickens. Yet the mechanisms of probiotic effects are not fully understood. This study has characterized our previously-selected […]

Clodronate treatment significantly depletes macrophages in chickens.

Clodronate treatment significantly depletes macrophages in chickens. Macrophages function as phagocytes and antigen-presenting cells in the body. As has been demonstrated in mammals, administration of clodronate [dichloromethylene bisphosphonate (Cl2MBP)] encapsulated liposomes results in depletion of macrophages. Although this compound has been used in chickens, its effectiveness in depleting macrophages has yet to be fully determined. […]

Induction of innate immune response following infectious bronchitis corona virus infection in the respiratory tract of chickens

Virology. 2014 Feb;450-451:114-21. doi: 10.1016/j.virol.2013.12.001. Epub 2013 Dec 30. Kameka AM1, Haddadi S1, Kim DS1, Cork SC1, Abdul-Careem MF2. Abstract Infectious bronchitis virus (IBV) replicates in the epithelial cells of trachea and lungs of chicken, however the mechanism of generation of innate immune response against IBV infection in these tissues has not been fully characterized. […]

Assessment of attenuated Salmonella vaccine strains in controlling experimental Salmonella Typhimurium infection in chickens.

Pei Y1, Parreira VR1, Roland KL1, Curtiss R 3rd1, Prescott JF1. Abstract in English, French Salmonella hold considerable promise as vaccine delivery vectors for heterologous antigens in chickens. Such vaccines have the potential additional benefit of also controlling Salmonella infection in immunized birds. As a way of selecting attenuated strains with optimal immunogenic potential as […]

TLR ligands induce antiviral responses in chicken macrophages.

Abstract Chicken macrophages express several receptors for recognition of pathogens, including Toll-like receptors (TLRs). TLRs bind to pathogen-associated molecular patterns (PAMPs) derived from bacterial or viral pathogens leading to the activation of macrophages. Macrophages play a critical role in immunity against viruses, including influenza viruses. The present study was designed to test the hypothesis that […]

Effects of ligands for Toll-like receptors 3, 4, and 21 as adjuvants on the immunogenicity of an avian influenza vaccine in chickens.

Viral Immunol. 2014 May;27(4):167-73. doi: 10.1089/vim.2013.0124. Epub 2014 May 5. St Paul M1, Barjesteh N, Brisbin JT, Villaneueva AI, Read LR, Hodgins D, Nagy É, Sharif S. Abstract Avian influenza viruses (AIV) are of great concern to the worldwide community as well as the poultry industry. Although existing vaccines are successful in limiting the spread […]

Avian influenza virus vaccines containing Toll-like receptors 2 and 5 ligand adjuvants promote protective immune responses in chickens.

Viral Immunol. 2014 May;27(4):160-6. doi: 10.1089/vim.2013.0129. Epub 2014 May 5. St Paul M1, Brisbin JT, Barjesteh N, Villaneueva AI, Parvizi P, Read LR, Nagy E, Sharif S. Abstract Vaccination remains a useful means for the control of avian influenza viruses (AIV) in chickens. Current vaccines can protect chickens from morbidity and mortality. However, they do […]