Cytokine Gene Expression in Lung Mononuclear Cells of Chickens Vaccinated with Herpesvirus of Turkeys and Infected with Marek’s Disease Virus

Viral Immunol. 2015 Nov;28(9):538-43. doi: 10.1089/vim.2015.0054. Epub 2015 Oct 8. Parvizi P1, Brisbin JT1, Read LR1, Sharif S1. Abstract Marek’s disease virus (MDV) enters the chicken host through the respiratory system. However, little is known about the host immune responses induced by MDV in the lungs. To characterize these responses, chickens were vaccinated with herpesvirus […]

Reduction of avian influenza virus shedding by administration of Toll-like receptor ligands to chickens

Vaccine. 2015 Sep 11;33(38):4843-9. doi: 10.1016/j.vaccine.2015.07.070. Epub 2015 Aug 1. Barjesteh N1, Shojadoost B1, Brisbin JT2, Emam M1, Hodgins DC1, Nagy É1, Sharif S3. Abstract Avian influenza viruses (AIV) are of concern to the poultry industry. Outbreaks of AIV highlight the urgent need for effective control measures. Prophylactic strategies should be explored that rapidly elicit […]

Systemic immune responses to an inactivated, whole H9N2 avian influenza virus vaccine using class B CpG oligonucleotides in chickens

Vaccine. 2015 Jul 31;33(32):3947-52. doi: 10.1016/j.vaccine.2015.06.043. Epub 2015 Jun 16. Singh SM1, Alkie TN2, Hodgins DC3, Nagy É4, Shojadoost B5, Sharif S6. Abstract Commercial vaccines against avian influenza viruses (AIV) in chickens consist mainly of inactivated AIV, requiring parenteral administration and co-delivery of an adjuvant. Limitations in T helper 1 or T helper 2 biased […]

Evaluation of a polysaccharide conjugate vaccine to reduce colonization by Campylobacter jejuni in broiler chickens

BMC Res Notes. 2015 Jun 2;8:204. doi: 10.1186/s13104-015-1203-z. Hodgins DC1, Barjesteh N2, St Paul M3,4, Ma Z5, Monteiro MA6, Sharif S7. Abstract BACKGROUND: Campylobacter jejuni is a leading bacterial cause of food-borne illness in humans. Symptoms range from mild gastroenteritis to dysentery. Contaminated chicken meat is the most common cause of infection. Broiler chickens become […]

TLR ligands induce antiviral responses in chicken macrophages.

Abstract Chicken macrophages express several receptors for recognition of pathogens, including Toll-like receptors (TLRs). TLRs bind to pathogen-associated molecular patterns (PAMPs) derived from bacterial or viral pathogens leading to the activation of macrophages. Macrophages play a critical role in immunity against viruses, including influenza viruses. The present study was designed to test the hypothesis that […]

Effects of ligands for Toll-like receptors 3, 4, and 21 as adjuvants on the immunogenicity of an avian influenza vaccine in chickens.

Viral Immunol. 2014 May;27(4):167-73. doi: 10.1089/vim.2013.0124. Epub 2014 May 5. St Paul M1, Barjesteh N, Brisbin JT, Villaneueva AI, Read LR, Hodgins D, Nagy É, Sharif S. Abstract Avian influenza viruses (AIV) are of great concern to the worldwide community as well as the poultry industry. Although existing vaccines are successful in limiting the spread […]

Avian influenza virus vaccines containing Toll-like receptors 2 and 5 ligand adjuvants promote protective immune responses in chickens.

Viral Immunol. 2014 May;27(4):160-6. doi: 10.1089/vim.2013.0129. Epub 2014 May 5. St Paul M1, Brisbin JT, Barjesteh N, Villaneueva AI, Parvizi P, Read LR, Nagy E, Sharif S. Abstract Vaccination remains a useful means for the control of avian influenza viruses (AIV) in chickens. Current vaccines can protect chickens from morbidity and mortality. However, they do […]

The effects of administration of ligands for Toll-like receptor 4 and 21 against Marek’s disease in chickens.

Vaccine. 2014 Apr 7;32(17):1932-8. doi: 10.1016/j.vaccine.2014.01.082. Epub 2014 Feb 13. Parvizi P1, Abdul-Careem MF2, Mallick AI3, Haq K4, Haghighi HR1, Orouji S1, Heidari M5, Behboudi S6, Sharif S7. Abstract Ligands for Toll-like receptors (TLRs) are known to stimulate immune responses, leading to protection against bacterial and viral pathogens. Here, we aimed to examine the effects […]

TLR ligands induce antiviral responses in chicken macrophages

Barjesteh N, Behboudi S, Brisbin JT, Villanueva AI, Nagy E, Sharif S ——————————————————————————————————- Chicken macrophages express several receptors for recognition of pathogens, including Toll-like receptors (TLRs). TLRs bind to pathogen-associated molecular patterns (PAMPs) derived from bacterial or viral pathogens leading to the activation of macrophages. Macrophages play a critical role in immunity against viruses, including […]

Immunostimulatory properties of Toll-like receptor ligands in chickens.

St Paul M, Brisbin JT, Abdul-Careem MF and Sharif S. Vet Immunol Immunopathol. 152 (2013):191-199. Abstract Toll-like receptors (TLRs) are evolutionarily conserved pattern recognition receptors that have been identified in mammals and avian species. Ligands for TLRs are typically conserved structural motifs of microorganisms termed pathogen-associated molecular patterns (PAMPs). Several TLRs have been detected in […]

Cytokine patterns associated with a serotype 8 fowl adenovirus infection.

Grgić H, Sharif S, Haghighi HR and Nagy É. Viral Immunol. 2 (2013):143-149. Abstract This study examined cytokine gene expression patterns associated with fowl adenovirus (FAdV) infection. The selected cytokine mRNA was quantified by quantitative real-time reverse transcription-PCR in spleen, liver, and cecal tonsil during the course of infection of chickens with a serotype 8 FAdV (FAdV-8). Compared […]

Small Interfering RNA-Mediated Knockdown of Chicken Interferon-γ Expression.

Haq K, Wootton SK, Barjesteh N, St Paul M, Golovan S, Bendall AJ and Sharif S. J Interferon Cytokine Res. 6 (2013):319-327. Abstract Interferon (IFN)-γ is a cytokine with a variety of functions, including direct antiviral activities and the capacity to polarize T-cells. However, there is limited information available about the function of this cytokine in the avian immune system. To […]

Induction of chicken cytokine responses in vivo and in vitro by lipooligosaccharide of Campylobacter jejuni HS:10.

Barjesteh N, Hodgins DC, St Paul M, Quinteiro-Filho WM, DePass C, Monteiro MA and Sharif S. Vet Microbiol. 164 (2013):122-130. Abstract Campylobacter jejuni is a pathogen of the gastrointestinal tract of humans, but colonizes chickens for prolonged periods without causing disease. It is unclear what host and bacterial mechanisms maintain a non-inflammatory state in chickens. The present work was undertaken to characterize […]

Characterization of chicken thrombocyte responses to Toll-like receptor ligands

St Paul M, Paolucci S, Barjesteh N, Wood RD, Schat KA and Sharif S.  PLoS One. 7:8 (2012):e43381.   Abstract Thrombocytes are the avian equivalent to mammalian platelets. In addition to their hemostatic effects, mammalian platelets rely in part on pattern recognition receptors, such as the Toll-like receptors (TLR), to detect the presence of pathogens […]

Vaccination with CpG-adjuvanted avian influenza virosomes promotes antiviral immune responses and reduces virus shedding in chickens.

Mallick AI, Kulkarni RR, St Paul M, Parvizi P, Nagy E, Behboudi S and Sharif S.  Viral Immunol. 25:3 (2012):226-31. Abstract The use of virosomes as a vaccine platform has proven successful against several viruses. Here we examined the protective efficacy of a virosome-based vaccine consisting of avian influenza virus (AIV) A/Duck/Czech/56/H4N6 in chickens against […]

Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens.

Kulkarni, R., Parreira, V., Sharif, S., Prescott, J. F. 2008. Vaccine 26:4194-4203. Abstract Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens but currently no effective vaccine is available. Our previous study showed that certain C. perfringens secreted proteins when administered intramuscularly protected chickens against experimental infection. In the current study, genes encoding […]